Histomorphological and histomorphometric changes in the testes of male Wistar rats following prepubertal exposure to caffeine

This experiment was designed to study the effects of caffeine on histomorphology testes prepubertal male Wistar rats. Prepubertal rats (25-28 days old), weighing between 50-60 g were randomly divided into three treatment groups (B, C and D) control (A) ten each. Group A received distilled water while Groups B, C, D respectively given daily dose 50 mg/kg, 100 200 mg/kg dissolved in for 14 by gavage. Five from each group sacrificed 24 hours after last administration remaining monitored another weeks them attain sexual maturity. The under diethyl ether anesthesia fixed immersion Bouin’s fluid. tissues processed histomorphological histomorphometric assessment. Data obtained analyzed using one way ANOVA followed Dunnett post hoc test comparison with control. Alpha level set at 0.05. Results showed that caused a significant decrease testicular weight marked cellular degeneration depletion germ cells D. height germinal epithelium significantly reduced when compared concluded exposure growth deleteriously alters morphology.